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Image Search Results
Journal: Journal of Anesthesia and Translational Medicine
Article Title: Spinal TREM2/PI3K/Akt signal axis elicits microglial activation and aggravates bone cancer pain
doi: 10.1016/j.jatmed.2025.04.002
Figure Lengend Snippet: Fig. 4. Functional blockade of TREM2 and PI3K expression reduces pathological activation of spinal microglia and the expression levels of inflammatory cytokines in male mice with bone cancer pain. (A) Representative Western blot and summary data of Iba-1 in the L3–L5 spinal cord tissues in the sham group, BCP group, shRNA- control group, and shRNA-TREM2 group, **P < 0.01, ***P < 0.001 vs. corresponding groups, n = 3 per group. (B) Representative Western blot and summary data of Iba-1 in the L3–L5 spinal cord tissues from male mice in the sham group, BCP group, LY294002-control group, and BCP-LY294002 group, *P < 0.05, **P < 0.01 vs. corresponding groups, n = 3 per group. (C–F) Using QPCR analysis to assess the expression of inflammatory cytokines IL-6 and IL-1β in the L3–L5 spinal cord tissues of male mice in relevant groups, *P < 0.05, **P < 0.01, ***P < 0.001, n = 3 per group. (G–K) The expression levels of inflammatory cytokines IL-6 and IL- 1β in the peripheral blood from male mice in the relevant groups were measured using ELISA. Statistical significance is denoted as *P < 0.05, **P < 0.01, ***P < 0.001, n = 5 per group. All data are presented as mean ± SEM. One-way ANOVA was used for statistical analysis. Abbreviations: BCP, bone cancer pain; Iba-1, ionized calcium binding adaptor molecule-1.
Article Snippet: Peripheral blood was expeditiously extracted from the orbit of profoundly anaesthetized mice, permitted to sit for a period, and subsequently centrifuged to obtain the supernatant.The levels of IL-6 and IL-1β were quantitated employing an enzyme-linked
Techniques: Functional Assay, Expressing, Activation Assay, Western Blot, shRNA, Control, Enzyme-linked Immunosorbent Assay, Binding Assay